Name : Human JAML Protein
Product Source :
Recombinant Human JAML Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu20-Leu275.[Accession | NP_001091996.1]
Molecular Weight :
The protein has a predicted MW of 30.1 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human JAML on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human JAML is greater than 95% as determined by SEC-HPLC.
Background :
JAML (junctional adhesion molecule-like), contains 2 extracellular immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic tail. JAML mRNA is expressed in hematopoietic tissues and is prominently expressed in granulocytes. The fact that JAML protein is localized at the cell plasma membrane in the areas of cell-cell contacts, whereas it is not detected at free cell borders, suggests that JAML is engaged in homophilic interactions.
Synonyms :
JunctioJAML; AMICA1; AMICA; CREA7-1; Gm638; JAML
References & Citations :
(1) Moog-Lutz C, Cavé-Riant F, Guibal FC, Breau MA, Di Gioia Y, Couraud PO, Cayre YE, Bourdoulous S, Lutz PG. JAML, a novel protein with characteristics of a junctional adhesion molecule, is induced during differentiation of myeloid leukemia cells. Blood. 2003 Nov 1;102(9):3371-8. doi: 10.1182/blood-2002-11-3462. Epub 2003 Jul 17. PMID: 12869515.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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