Name : Human KLRG1 Protein
Product Source :
Recombinant Human KLRG1 Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Leu60-Phe195.[Accession | Q96E93-1]
Molecular Weight :
The protein has a predicted MW of 42.8 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human KLRG1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human KLRG1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human KLRG1, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-KLRG1 Antibody, hFc Tag with the EC50 of 20.8ng/ml determined by ELISA.
Background :
Immune homeostasis requires the tight, tissue-specific control of the different CD4 Foxp3 regulatory T (Treg) cell populations. The cadherin-binding inhibitory receptor killer cell lectin-like receptor G1 (KLRG1) is expressed by a subpopulation of Treg cells with GATA3 effector phenotype.Lack of KLRG1 on Treg cells conferred on them a competitive advantage in the gut, but not in lymphoid organs. Hence, although absence of KLRG1 is not enough to increase intestinal Treg cells in KLRG1 knockout mice, KLRG1 ligation reduces T-cell receptor signals and the competitive fitness of individual Treg cells in the intestine.
Synonyms :
KLRG1; CLEC15A; MAFA; MAFAL; 2F1; CLEC15AMAFA-LIKE; MGC13600
References & Citations :
(1) Meinicke H, Bremser A, Brack M, Schrenk K, Pircher H, Izcue A. KLRG1 impairs regulatory T-cell competitive fitness in the gut. Immunology. 2017 Sep;152(1):65-73. doi: 10.1111/imm.12749. Epub 2017 Jun 20. PMID: 28437578; PMCID: PMC5543461.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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