Name : Biotinylated Human KIR3DL3 Protein
Product Source :
Recombinant Biotinylated Human KIR3DL3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu26-Leu322.[Accession | Q8N743]
Molecular Weight :
The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 48-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl, 0.2M L-arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human KIR3DL3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human KIR3DL3 is greater than 95% as determined by SEC-HPLC. SPR Data Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human KIR3DL3, His-Avi Tag with an affinity constant of 0.254 μM as determined in SPR assay (Biacore T200).
Background :
Killer-cell immunoglobulin-like receptor (KIR) 3DL3 is a framework gene present in all human KIR haplotypes.KIR3DL3 has been shown to be constitutively expressed at a low RNA level in peripheral blood mononuclear cell (PBMC) and decidual natural kill (NK) cells.
Synonyms :
CD158Z; KIR2DS2; KIR3DL7; KIR44; KIRC1; KIR3DL3
References & Citations :
(1)Jones DC, Hiby SE, Moffett A, Trowsdale J, Young NT. Nature of allelic sequence polymorphism at the KIR3DL3 locus. Immunogenetics. 2006;58(8):614-627. doi:10.1007/s00251-006-0130-5
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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