Name : Canine keratin, type I cytoskeletal 19 (310-399) Protein
Product Source :
Recombinant Canine keratin, type I cytoskeletal 19(310-399) Protein is expressed from E.coli with His tag at the N-Terminus. It contains Gln310-Leu399.[Accession | NP_001240671.1]
Molecular Weight :
The protein has a predicted MW of 21.84 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Canine keratin, type I cytoskeletal 19 (310-399) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Canine keratin, type I cytoskeletal 19 (310-399) is greater than 95% as determined by SEC-HPLC.
Background :
Keratin 19 (K19) belongs to the keratin family of proteins, which maintains structural integrity of epithelia. In cancer, K19 is highly expressed in several types where it serves as a diagnostic marker.
Synonyms :
Cytokeratin-19; Cytokeratin19; CK-19; CK19; Keratin-19; Keratin19; K19; KRT19
References & Citations :
Sharma P, Alsharif S, Bursch K, Parvathaneni S, Anastasakis DG, Chahine J, Fallatah A, Nicolas K, Sharma S, Hafner M, Kallakury B, Chung BM. Keratin 19 regulates cell cycle pathway and sensitivity of breast cancer cells to CDK inhibitors. Sci Rep. 2019 Oct 10;9(1):14650. doi: 10.1038/s41598-019-51195-9. PMID: 31601969; PMCID: PMC6787034.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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