Name : Rhesus macaque LILRB1/CD85j/ILT2 Protein
Product Source :
Recombinant Rhesus macaque LILRB1/CD85j/ILT2 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Met1-His474.[Accession | NP_001035762.2]
Molecular Weight :
The protein has a predicted MW of 75.2 kDa. Due to glycosylation, the protein migrates to 80-90 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Rhesus macaque LILRB1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Rhesus macaque LILRB1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 0.17μg/ml determined by ELISA (QC Test). SPR Data Rhesus macaque LILRB1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag with an affinity constant of 1.74 nM as determined in SPR assay (Biacore T200). SPR Data Anti-LILRB1 Antibody immobilized on CM5 Chip can bind Rhesus macaque LILRB1, hFc Tag with an affinity constant of 47.69 nM as determined in SPR assay (Biacore T200).
Background :
LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response.
Synonyms :
ILT2; ILT-2; ILT2FLJ37515; LILRB1; LIR1; MIR7; CD85J; XXbac-BCX85G21.4
References & Citations :
(1)Apps R , Gardner L , Sharkey A , et al. A homodimeric complex of HLA-G on normal trophoblast cells modulates antigen-presenting cells via LILRB1 [J]. European Journal of Immunology, 2007, 37(7):1924-1937.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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