Name : Human LRP-5 Protein
Product Source :
Recombinant Human LRP-5 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Glu644-Gln1263.[Accession | O75197-1]
Molecular Weight :
The protein has a predicted MW of 96.51 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Supplied as 0.22μm filtered solution in PBS, 100mM L-arginine (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human LRP-5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human DKK1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human LPR-5, hFc Tag with the EC50 of 1.04μg/ml determined by ELISA (QC Test). SPR Data Human LRP-5, hFc Tag captured on CM5 Chip via Protein A can bind Human DKK1, His Tag with an affinity constant of 0.28 μM as determined in SPR assay (Biacore T200).
Background :
A role for low-density lipoprotein-related receptor 5 (LRP5) in human bone was first established by the identification of genetic alterations that led to dramatic changes in bone mass. Shortly thereafter, mutations that altered the function of the sclerostin (SOST) gene were also associated with altered human bone mass. Subsequent studies of LRP5 and sclerostin have provided important insights into the mechanisms by which these proteins regulate skeletal homeostasis.
Synonyms :
LRP-5; LRP-7; LR3; BMND1; BMND1OPTA1; EVR1; EVR4; HBM; LR3VBCH2; LRP5; LRP7; OPPG; OPS; OPTA1; VBCH2
References & Citations :
(1)Williams BO. LRP5: From bedside to bench to bone. Bone. 2017 Sep;102:26-30. doi: 10.1016/j.bone.2017.03.044. Epub 2017 Mar 21. PMID: 28341377.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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