Name : Human LRP-6 Protein
Product Source :
Recombinant Human LRP-6 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus. It contains Ala20-Pro630.[Accession | O75581-1]
Molecular Weight :
The protein has a predicted MW of 95.3 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 90% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human LRP-6 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%. ELISA Data Immobilized Biotinylated Human SOST, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 0.17μg/ml determined by ELISA.
Background :
Dickkopf-related protein 1 (DKK-1), a ligand for the WNT coreceptors low-density lipoprotein receptor-related proteins 5 and 6 (LRP-5 and LRP-6) and an inhibitor of WNT/β-catenin signaling, effectively inhibits pericyte activation, detachment, and transition to myofibroblasts in vivo in response to kidney injury, resulting in attenuated fibrogenesis, capillary rarefaction, and inflammation.LRP-6 interacts closely with PDGF receptor β and TGF-β receptor 1 at the cell membrane, suggesting that it may have roles in pathways other than WNT/β-catenin.
Synonyms :
LRP-6; DCAD2; FLJ90062; FLJ90421;LRP6
References & Citations :
(1) Ren S, Johnson BG, Kida Y, Ip C, Davidson KC, Lin SL, Kobayashi A, Lang RA, Hadjantonakis AK, Moon RT, Duffield JS. LRP-6 is a coreceptor for multiple fibrogenic signaling pathways in pericytes and myofibroblasts that are inhibited by DKK-1. Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1440-5. doi: 10.1073/pnas.1211179110. Epub 2013 Jan 9. Erratum in: Proc Natl Acad Sci U S A. 2016 Nov 14;: PMID: 23302695; PMCID: PMC3557093.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
PILR-alpha Protein
IL-17RA Protein
Popular categories:
CEACAM1
VRK Serine/Threonine Kinase 1