Name : Human LTBR Protein
Product Source :
Recombinant Human LTBR Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gln31-Met227.[Accession | P36941-1]
Molecular Weight :
The protein has a predicted MW of 48.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human LTBR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human LTBR is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human LTBR, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-LTBR Antibody, hFc Tag with the EC50 20.7ng/ml determined by ELISA (QC Test). ELISA Data Immobilized Human LIGHT, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human LTBR, hFc Tag with the EC50 22.9ng/ml determined by ELISA.
Background :
Components of lymphotoxin beta receptor (LTBR)-associated signaling complexes, including TRAF2, TRAF3, NIK, IKK1, and IKK2 have been shown to participate in the coupling of LTBR to NFkappaB.TRAF3 functions as a negative regulator of LTBR signaling via both canonical and non-canonical NFkappaB pathways by two distinct mechanisms. These effects of TRAF3 depletion did not require LTBR signaling and were consistent with autonomous activation of the non-canonical NFkappaB pathway.
Synonyms :
TNFCR; TNFR3; TNFRSF3; LTBR; CD18; TNFR2-RP; TNFR-RP; LT-BETA-R; TNF-R-III; D12S370; LT-β-R
References & Citations :
(1) Bista P, Zeng W, Ryan S, Bailly V, Browning JL, Lukashev ME. TRAF3 controls activation of the canonical and alternative NFkappaB by the lymphotoxin beta receptor. J Biol Chem. 2010 Apr 23;285(17):12971-8. doi: 10.1074/jbc.M109.076091. Epub 2010 Feb 25. PMID: 20185819; PMCID: PMC2857099.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
C1QA Protein
CRELD2 Protein
Popular categories:
NCA-95/CD66b
IFN-alpha 13