Name : Human ACE2/ACEH Protein
Product Source :
Recombinant Human ACE2/ACEH Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln18-Ser740.[Accession | Q9BYF1-1]
Molecular Weight :
The protein has a predicted MW of 84.7 kDa. Due to glycosylation, the protein migrates to 85-110 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human ACE2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human ACE2 is greater than 95% as determined by SEC-HPLC ELISA Data Immobilized Human ACE2, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 46.2ng/ml determined by ELISA (QC Test). SPR Data SARS-COV-2 Spike RBD captured on Protein A chip, can bind Human ACE2, His Tag with an affinity constant of 11.9nM as determined in a SPR assay (Biacore T200).
Background :
ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension.The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.
Synonyms :
ACE-2; ACEH; ACE2
References & Citations :
(1)Kuba K , Imai Y , Rao S , et al. A crucial role of angiotensin converting enzyme 2 (ACE2) in SARS coronavirus–induced lung injury[J]. Nature Medicine, 2005, 11(8):875-879.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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