Name : Mouse MANSC1 Protein

Product Source :
Recombinant Mouse MANSC1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gly25-Leu369.[Accession | Q9CR33]

Molecular Weight :
The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 58-110 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Mouse MANSC1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse MANSC1 is greater than 95% as determined by SEC-HPLC.

Background :
MANSC1 contains 1 MANSC domain. MANSC is a seven-cysteine-containing domain present in animal membrane and extracellular proteins. MANSC (motif at N terminus with seven cysteines) is a novel domain with a well-conserved seven-cysteine motif that is present at the N terminus of membrane and extracellular proteins, including low-density lipoprotein receptor-related protein 11 (LRP-11), hepatocyte growth factor activator inhibitor 1 (HAI-1) and some uncharacterized proteins encoded by multicellular animals from Mollusca to Chordata. The MANSC domain in HAI-2 might function through binding with hepatocyte growth factor activator and matriptase[1].

Synonyms :
MANSC1; LOH12CR3; 9130403P13Rik

References & Citations :
(1)Guo J, Chen S, Huang C, Chen L, Studholme DJ, Zhao S, Yu L. MANSC: a seven-cysteine-containing domain present in animal membrane and extracellular proteins. Trends Biochem Sci. 2004 Apr;29(4):172-4.

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