Name : Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A4 (KVLEHVVRV) Monomer Protein
Product Source :
Recombinant Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A4 (KVLEHVVRV) Monomer Protein is expressed from HEK293 with His tag at the C-terminus. It contains Gly25-Thr206 (Human HLA-A*02:01 α1&α2) and Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M) and KVLEHVVRV peptide.[Accession | A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse H-2Ld α3)&P01887(Mouse B2M)&KVLEHVVRV]
Molecular Weight :
The protein has a predicted MW of 48.00 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A4 (KVLEHVVRV) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A4 (KVLEHVVRV) Monomer is greater than 95% as determined by SEC-HPLC. SPR Data HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) TCR immobilized on CM5 Chip can bind Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A4 (KVLEHVVRV) Monomer, His Tag with an affinity constant of 4.76 μM as determined in SPR assay (Biacore T200).
Background :
MAGE-A4 and MAGE-A8 are type I membes of the melanoma associated antigen (MAGE) family. The MAGE family is a large, highly conserved group of proteins that share a common MAGE homology domain. Both MAGE-A4 and MAGE-A8 antigen-presenting peptides can be presented by HLA-A*02:01.
Synonyms :
MAGE-A4; MAGE-A8;
References & Citations :
(1) Weon JL, Potts PR. The MAGE protein family and cancer. Curr Opin Cell Biol. 2015 Dec;37:1-8. doi: 10.1016/j.ceb.2015.08.002. Epub 2015 Sep 3. PMID: 26342994; PMCID: PMC4688208.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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