Name : Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein
Product Source :
Recombinant Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and GVYDGREHTV peptide.[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&GVYDGREHTV]
Molecular Weight :
The protein has a predicted MW of 50.5 kDa. Due to glycosylation,the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA.
Background :
Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and GVYDGREHTV peptide of the MAGE-A4.
Synonyms :
HLA0201; MHC I; MAGE-A4; CT1.4; MAGE4A; MAGE4B; MAGE-X2; member 4
References & Citations :
(1)Kageyama S , Ikeda H , Miyahara Y , et al. Adoptive Transfer of MAGE-A4 T-cell Receptor Gene-Transduced Lymphocytes in Patients with Recurrent Esophageal Cancer[J]. Clinical Cancer Research, 2015, 21(10):2268.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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