Name : Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Monomer Protein

Product Source :
Recombinant Human HBV(HLA-A*02:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and FLLTRILTI peptide.[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&FLLTRILTI peptide]

Molecular Weight :
The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Monomer is greater than 95% as determined by SEC-HPLC.

Background :
Hepatitis B virus (HBV), is the leading cause of liver diseases infecting an estimated 240 million persons worldwide. The HBV prevalence rates are variables between different countries, with an high level of endemicity in the south-eastern part of Europe. Seven main HBV-D subgenotypes have been described until now (D1-D7).

Synonyms :
Hepatitis B virus; MHC; HBV

References & Citations :
Ciccozzi M, Ciccaglione AR, Lo Presti A, Equestre M, Cella E, Ebranati E, Gabanelli E, Villano U, Bruni R, Yalcinkaya T, Tanzi E, Zehender G. Evolutionary dynamics of HBV-D1 genotype epidemic in Turkey. J Med Virol. 2014 Jan;86(1):109-16. doi: 10.1002/jmv.23740. Epub 2013 Oct 24. PMID: 24243521.

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