Name : Biotinylated Human BAFFR/TNFRSF13C Protein
Product Source :
Recombinant Biotinylated Human BAFFR/TNFRSF13C Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ser7-Ala71.[Accession | Q96RJ3-1]
Molecular Weight :
The protein has a predicted MW of 9.5 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human BAFFR on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. ELISA Data Immobilized Human BAFF (Trimer) , His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human BAFFR, His Tag with the EC50 of 53.5ng/ml determined by ELISA (QC Test). ELISA Data Immobilized Anti-BAFFR Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human BAFFR, His Tag with the EC50 of 5.5ng/ml determined by ELISA.
Background :
BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF Receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF.
Synonyms :
CD268; BAFFR; BR3; TNFRSF13C; BROMIX; CVID4; prolixin
References & Citations :
(1)Tin K , Peng C , Christopher T , et al. BAFF Receptor mAb Treatment Ameliorates Development and Progression of Atherosclerosis in Hyperlipidemic ApoE?/? Mice[J]. PLoS ONE, 2013, 8(4):e60430-.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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