Name : HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein
Product Source :
Recombinant HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ELAGIGILTV peptide.[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&ELAGIGILTV]
Molecular Weight :
The protein has a predicted MW of 50.40 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer is greater than 95% as determined by SEC-HPLC.
Background :
Melanoma antigen recognized by T cell-1 (Mart-1), one of the melanosome-specific proteins, also recognized by cytotoxicity T lymphocytes as a marker. Mart-1 is considered to play a critical role in the immunotherapy for melanoma.
Synonyms :
MART1; LB39-AA; SK29-AA; Melan-A; MLANA
References & Citations :
Wang J, Jia R, Yao Y, Cun B, Huang X, Gu P, Ge S, Fan X. Differential expression of Mart-1 in human uveal melanoma cells. Mol Med Rep. 2011 Sep-Oct;4(5):799-803. doi: 10.3892/mmr.2011.504. Epub 2011 Jun 7. PMID: 21667026.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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