Name : Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Monomer Protein
Product Source :
Recombinant Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and KVLEHVVRV peptide.[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&KVLEHVVRV]
Molecular Weight :
The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Monomer is greater than 95% as determined by SEC-HPLC.
Background :
MAGE-A4 and MAGE-A8 are type I membes of the melanoma associated antigen (MAGE) family. The MAGE family is a large, highly conserved group of proteins that share a common MAGE homology domain. Both MAGE-A4 and MAGE-A8 antigen-presenting peptides can be presented by HLA-A*02:01.
Synonyms :
MAGE-A4;
References & Citations :
(1) Weon JL, Potts PR. The MAGE protein family and cancer. Curr Opin Cell Biol. 2015 Dec;37:1-8. doi: 10.1016/j.ceb.2015.08.002. Epub 2015 Sep 3. PMID: 26342994; PMCID: PMC4688208.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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