Name : FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer Protein
Product Source :
Recombinant FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer Protein is expressed from HEK293 with His tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*03:01) and Ile21-Met119 (B2M).[Accession | NP_002107.3(HLA-A*03:01)&P61769(B2M)]
Molecular Weight :
The protein has a predicted MW of 301.2 kDa.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
Formulation :
Supplied as 0.22 μm filtered solution in PBS, 300mM NaCl (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
ELISA Data Immobilized Anti-HLA class I (W6/32) Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer, His Tag with the EC50 of 0.74μg/ml determined by ELISA.
Background :
Peptide Ready HLA-A*03:01&B2M Tetramer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*03:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
Synonyms :
MHC; HLA-A*03:01; Peptide Ready
References & Citations :
[1] Moritz A, Anjanappa R, Wagner C, Bunk S, Hofmann M, Pszolla G, Saikia A, Garcia-Alai M, Meijers R, Rammensee HG, Springer S, Maurer D. High-throughput peptide-MHC complex generation and kinetic screenings of TCRs with peptide-receptive HLA-A*02:01 molecules. Sci Immunol. 2019 Jul 19;4(37):eaav0860.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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