Name : Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer Protein
Product Source :
Recombinant Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KMVELVHFL peptide.[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&KMVELVHFL]
Molecular Weight :
The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer is greater than 95% as determined by SEC-HPLC.
Background :
Melanoma antigen gene (MAGE)-A2 (MAGEA2) is a member of the MAGE-A family proteins widely studied for cancer vaccine development and identification of tumor markers. MAGEA2 plays an oncogenic role in glioma progression, and they provide insight into MAGEA2 application as a novel predictor of clinical outcomes and a potential glioma biomarker.
Synonyms :
MAGE-A2;MAGEA2
References & Citations :
Meng Q, Luo G, Liu B, Sun Y, Yan Z. Melanoma-associated antigen A2 is overexpressed in glioma and associated with poor prognosis in glioma patients. Neoplasma. 2018;65(4):604-609. doi: 10.4149/neo_2018_170625N440. PMID: 30064232.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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