Name : Biotinylated Human 4-1BB/TNFRSF9 Protein
Product Source :
Recombinant Biotinylated Human 4-1BB/TNFRSF9 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu24-Gln186.[Accession | Q07011]
Molecular Weight :
The protein has a predicted MW of 20.2 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human 4-1BB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human 4-1BB is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human 4-1BB Ligand (Trimer), His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human 4-1BB, His Tag with the EC50 of 0.16μg/ml determined by ELISA.
Background :
4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation.
Synonyms :
CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA
References & Citations :
(1)Vinay D S , Kwon B S. 4-1BB (CD137), an inducible costimulatory receptor, as a specific target for cancer therapy[J]. BMB Reports, 2014, 47(3):122-129.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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