Name : Biotinylated Human HLA-A*11:01&B2M&HPV16 E7 (IVCPICSQK) Monomer Protein
Product Source :
Recombinant Biotinylated Human HLA-A*11:01&B2M&HPV16 E7 (IVCPICSQK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305 (HLA-A*11:01), Ile21-Met119 (B2M) and IVCPICSQK peptide.[Accession | AAV53343.1(HLA-A*11:01)&P61769(B2M)&IVCPICSQK]
Molecular Weight :
The protein has a predicted MW of 50.39 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human HLA-A*11:01&B2M&HPV16 E7 (IVCPICSQK) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human HLA-A*11:01&B2M&HPV16 E7 (IVCPICSQK) Monomer is greater than 95% as determined by SEC-HPLC.
Background :
HPV16 E7 protein, one of the primary target proteins in molecular targeted therapy for HPV-induced cervical cancer. The affitoxin, ZHPV16E7 affitoxin384 was generated by fusing the modified Pseudomonas Exotoxin A (PE38KDEL) to the HPV16 E7-specific affibody.
Synonyms :
HPV16; E7; MHC;
References & Citations :
Jiang P, Wang L, Hou B, Zhu J, Zhou M, Jiang J, Wang L, Chen S, Zhu S, Chen J, Zhang L. A novel HPV16 E7-affitoxin for targeted therapy of HPV16-induced human cervical cancer. Theranostics. 2018 Jun 7;8(13):3544-3558. doi: 10.7150/thno.24607. PMID: 30026865; PMCID: PMC6037027.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
UBE2I Protein
TNF-alpha/TNFSF2 Protein
Popular categories:
Leukocyte Elastase Inhibitor
Caspase-1