Name : Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer Protein
Product Source :
Recombinant Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer Protein is expressed from HEK293 with His tag at the C-terminus. It contains Gly25-Thr206 (Human HLA-A*02:01 α1&α2), Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M) and RMFPNAPYL peptide.[Accession | A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse H-2Ld α3)&P01887(Mouse B2M)&RMFPNAPYL]
Molecular Weight :
The protein has a predicted MW of 48.04 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer, His Tag with the EC50 of 39.6ng/ml determined by ELISA.
Background :
The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and RMFPNAPYL peptide of the WT-1.
Synonyms :
MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33
References & Citations :
(1)Dao T , Pankov D , Scott A , et al. Therapeutic bispecific T-cell engager antibody targeting the intracellular oncoprotein WT1[J]. Nature Biotechnology, 2015.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
BAFFR/TNFRSF13C Protein
Animal-Free IL-12 alpha Protein
Popular categories:
PTPN2
VCAM-1/CD106