Name : Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer Protein
Product Source :
Recombinant Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains His23-Pro296(Qa-1b), Ile21-Met119(B2M) and AMAPRTLLL peptide.[Accession | P06339(Q1-1b)&P01887(B2M)&AMAPRTLLL]
Molecular Weight :
The protein has a predicted MW of 50.60 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer is greater than 95% as determined by SEC-HPLC.
Background :
Qa-1b binds a peptide (AMAPRTLLL), referred to as Qdm (for Qa-1 determinant modifier), derived from the signal sequence of murine class Ia molecules. This peptide binds with high affinity and accounts for almost all of the peptides associated with this molecule. Human histocompatibility leukocyte antigen (HLA)-E, a homologue of Qa-1b, binds similar peptides derived from human class Ia molecules and interacts with CD94/NKG2 receptors on natural killer cells.
Synonyms :
Qdm; Qa-1b; MHC; H2-T23
References & Citations :
Kurepa Z, Hasemann CA, Forman J. Qa-1b binds conserved class I leader peptides derived from several mammalian species. J Exp Med. 1998 Sep 7;188(5):973-8. doi: 10.1084/jem.188.5.973. PMID: 9730898; PMCID: PMC2213384.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
Lactoferrin/LTF Protein
FSTL5 Protein
Popular categories:
ITIH3
CD29/Integrin beta-1