Name : Human MICB Protein
Product Source :
Recombinant Human MICB Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala23-Gly298.[Accession | Q29980-1]
Molecular Weight :
The protein has a predicted MW of 34.4 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human MICB on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Human MICB is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human MICB, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.62μg/ml determined by ELISA (QC Test). ELISA Data Immobilized Human MICB, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-MICB Antibody, hFc Tag with the EC50 of 42.4ng/ml determined by ELISA. SPR Data Human NKG2D, hFc Tag captured on CM5 Chip via Protein A can bind Human MICB, His Tag with an affinity constant of 83.31 nM as determined in SPR assay (Biacore T200).
Background :
MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Synonyms :
MICB; MIC-B; PERB11.2
References & Citations :
(1)Spear P , Wu M R , Sentman M L , et al. NKG2D ligands as therapeutic targets[J]. Cancer immunity: a journal of the Academy of Cancer Immunology, 2013, 13(2):8.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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