Name : Human c-MPL/Thrombopoietin R Protein
Product Source :
Recombinant Human c-MPL/Thrombopoietin R Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln26-Trp491.[Accession | P40238-1]
Molecular Weight :
The protein has a predicted MW of 53.6 kDa. Due to glycosylation, the protein migrates to 68-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human c-MPL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human c-MPL is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human c-MPL, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-c-MPL Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.
Background :
The c-mpl gene encodes a member of the hematopoietic cytokine receptor superfamily. This gene was discovered through the study of a murine retrovirus which induces an acute myeloproliferative syndrome in mice.MPLV (for myeloproliferative leukemia virus) has transduced a truncated and constitutively activated form of the c-mpl receptor chain. High levels of c-mpl expression were detected in 45% of acute myeloid leukemia (AML). No correlation was found between c-mpl expression and the French-American-British classification subtype of AML.
Synonyms :
TPO-R; CD110; MPL; c-Mpl; MPLV; K12
References & Citations :
(1) Bouscary D, Prudhomme C, Quesnel B, Melle J, Picard F, Dreyfus F. c-mpl expression in hematologic disorders. Leuk Lymphoma. 1995 Mar;17(1-2):19-26. doi: 10.3109/10428199509051699. PMID: 7773160.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
Serpin E1 Protein
AXL Protein
Popular categories:
CD3E-CD3G Heterodimer Proteins
B Lymphoid Tyrosine Kinase