Name : Biotinylated Human MSLN/Mesothelin Protein
Product Source :
Recombinant Biotinylated Human MSLN/Mesothelin Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Glu296-Gly580.[Accession | Q13421-2]
Molecular Weight :
The protein has a predicted MW of 61.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human MSLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human MSLN is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CA125, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human MSLN, hFc Tag with the EC50 of 44.1ng/ml determined by ELISA (QC Test). ELISA Data Immobilized Anti-MSLN Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human MSLN, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA. FACS Data Use Biotinylated Human MSLN-hFc-Avi protein to detect the expression rate of Anti-MSLN-CAR positive cell. 293T cells and anti-MSLN CAR-293T cells were incubated with Biotinylated human MSLN-hFc-Avi Tag. Non-transfected 293T cells and Fc-labeled protein control were used as negative control. SA-FITC was used to evaluate the binding activity of Biotinylated Human MSLN.
Background :
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.
Synonyms :
MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR
References & Citations :
(1)Adusumilli P S , Cherkassky L , Villena-Vargas J , et al. Regional delivery of mesothelin-targeted CAR T cell therapy generates potent and long-lasting CD4-dependent tumor immunity[J]. Science Translational Medicine, 2014, 6(261):261ra151-261ra151.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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