Name : Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling)
Product Source :
Recombinant Biotinylated Human 4-1BB Ligand/TNFSF9 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with monomeric hFc tag at the N-Terminus. It contains Arg71-Glu254.[Accession | P41273]
Molecular Weight :
The protein has a predicted MW of 84.3 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 90% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human 4-1BB Ligand (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human 4-1BB Ligand (Trimer) is greater than 90% as determined by SEC-HPLC. ELISA Data Immobilized Human 4-1BB, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA.
Background :
The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.
Synonyms :
41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9
References & Citations :
(1)Vinay D S , Kwon B S. 4-1BB (CD137), an inducible costimulatory receptor, as a specific target for cancer therapy[J]. BMB Reports, 2014, 47(3):122-129.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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