Name : Human MASP2 Protein
Product Source :
Recombinant Human MASP2 Protein is expressed from E.coli with His tag at the C-Terminus. It contains Gly288-Phe686.[Accession | O00187-1]
Molecular Weight :
The protein has a predicted MW of 46.9 kDa. Due to autocatalytic cleavage, the protein migrates to 18 kDa&25-30 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in 20mM Tris, 200mM NaCl (pH 9.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human MASP2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human MASP2, His Tag at 0.2μg/ml (100μl/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test). SPR Data Anti-MASP2 Antibody captured on CM5 Chip via Protein A can bind Human MASP2, His Tag with an affinity constant of 3.08 nM as determined in SPR assay (Biacore T200).
Background :
The pathogenesis of severe acute respiratory disease syndrome (SARS) is not fully understood. One case-control study has reported an association between susceptibility to SARS and mannan-binding lectin (MBL) in China. As the downstream protein of MBL, variants of the MBL-associated serine protease-2 (MASP2) gene may be associated with SARS coronavirus (SARS-CoV) infection in the same population.
Synonyms :
MAP19; MASP-2; MASP1P1; sMap
References & Citations :
(1) Wang Y, Yan J, Shi Y, Li P, Liu C, Ma Q, Yang R, Wang X, Zhu L, Yang X, Cao C. Lack of association between polymorphisms of MASP2 and susceptibility to SARS coronavirus infection. BMC Infect Dis. 2009 May 1;9:51. doi: 10.1186/1471-2334-9-51. PMID: 19405982; PMCID: PMC2683852.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
ADAM8 Protein
Serpin E2 Protein
Popular categories:
Cathepsin W
Retinoid X Receptor beta