Name : Human MASP3 Protein
Product Source :
Recombinant Human MASP3 Protein is expressed from HEK293 with His tag at the C-terminus. It contains Ile450-Val721.[Accession | P48740-2]
Molecular Weight :
The protein has a predicted MW of 31.10 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human MASP3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human MASP3 is greater than 95% as determined by SEC-HPLC. Bioactivity Data Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is > 10000 pmol/min/µg.
Background :
Mannose-binding lectin-associated serine protease-1 (also named MASP3), a multifunctional serine protease, plays an important role in innate immunity which is capable of activating the lectin pathway of the complement system and also triggering coagulation cascade system.
Synonyms :
MASP-3; MASP-1; MASP1; RaRF; Serine protease 5; CRARF; CRARF1; PRSS5;
References & Citations :
Mu L, Yin X, Wu H, Han K, Wu L, Ding M, Bian X, Li B, Fu S, Liang F, Guo Z, Ye J. Expression and functional characterization of a mannose-binding lectin-associated serine protease-1 (MASP-1) from Nile tilapia (Oreochromis niloticus) in host defense against bacterial infection. Fish Shellfish Immunol. 2019 Aug;91:68-77. doi: 10.1016/j.fsi.2019.05.014. Epub 2019 May 13. PMID: 31096060.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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