Name : Mouse MASP2 Protein
Product Source :
Recombinant Mouse MASP2 Protein is expressed from E.coli with His tag at the C-Terminus. It contains Thr287-Phe685.[Accession | Q91WP0]
Molecular Weight :
The protein has a predicted MW of 46.6 kDa. Due to autocatalytic cleavage, the protein migrates to 18-20 kDa&27-30 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22 μm filtered solution in 50mM Tris, 200mM NaCl (pH 9.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse MASP2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Mouse MASP2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA.
Background :
The dysregulation of complement cascade leads to unsolicited cytokine storm, inflammation, deterioration of alveolar lining cells, culminating in acquired respiratory destructive syndrome (ARDS). Similar pathogenesis is observed with the middle east respiratory syndrome (MERS), severe acquired respiratory syndrome (SARS), and SARS-CoV-2. Activation of the lectin pathway via mannose-binding lectin associated serine protease 2 (MASP2) is witnessed under discrete viral infections including COVID-19.
Synonyms :
MAP19; MASP-2; MASP1P1; sMap
References & Citations :
(1) Ram Kumar Pandian S, Arunachalam S, Deepak V, Kunjiappan S, Sundar K. Targeting complement cascade: an alternative strategy for COVID-19. 3 Biotech. 2020 Nov;10(11):479. doi: 10.1007/s13205-020-02464-2. Epub 2020 Oct 19. PMID: 33088671; PMCID: PMC7571295.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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