Name : Human MUC18/CD146 Protein
Product Source :
Recombinant Human MUC18/CD146 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Val24-Gly559.[Accession | P43121]
Molecular Weight :
The protein has a predicted MW of 60.8 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human MUC18/CD146 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human MUC18/CD146 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human MUC18, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MUC18, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA.
Background :
METCAM/MUC18 is an integral membrane cell adhesion molecule (CAM) in the Ig-like gene super-family. It can carry out common functions of CAMs which is to perform intercellular interactions and interaction of cell with extracellular matrix in tumor microenvironment, to interact with various signaling pathways and to regulate general behaviors of cells.The above positive correlation is fortuitous; actually METCAM/MUC18 acts as a tumor and metastasis suppressor for the ovarian carcinoma cells.
Synonyms :
Gicerin; CD146; MCAM; MUC18
References & Citations :
(1) Wu GJ. METCAM/MUC18 Decreases the Malignant Propensity of Human Ovarian Carcinoma Cells. Int J Mol Sci. 2018 Sep 29;19(10):2976. doi: 10.3390/ijms19102976. PMID: 30274262; PMCID: PMC6213002.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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