Name : Cynomolgus BCAM Protein
Product Source :
Recombinant Cynomolgus BCAM Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu32-Gly548.[Accession | A0A7N9CA99]
Molecular Weight :
The protein has a predicted MW of 57.42 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus BCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus BCAM is greater than 95% as determined by SEC-HPLC.
Background :
Lutheran/basal cell adhesion molecule (Lu/BCAM) is a transmembrane adhesion molecule expressed by erythrocytes and endothelial cells that can interact with the extracellular matrix protein laminin-α5. In sickle cell disease, Lu/BCAM is thought to contribute to adhesion of sickle erythrocytes to the vascular wall, especially during vaso-occlusive crises.
Synonyms :
LU; MSK19; Gplu; CD239; Lutheran;
References & Citations :
Klei TRL, de Back DZ, Asif PJ, Verkuijlen PJJH, Veldthuis M, Ligthart PC, Berghuis J, Clifford E, Beuger BM, van den Berg TK, van Zwieten R, El Nemer W, van Bruggen R. Glycophorin-C sialylation regulates Lu/BCAM adhesive capacity during erythrocyte aging. Blood Adv. 2018 Jan 3;2(1):14-24. doi: 10.1182/bloodadvances.2017013094. PMID: 29344581; PMCID: PMC5761629.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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