Name : Cynomolgus Nectin-4 Protein
Product Source :
Recombinant Cynomolgus Nectin-4 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gly32-Val351.[Accession | XP_005541277.1]
Molecular Weight :
The protein has a predicted MW of 35.9 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus Nectin-4 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus Nectin-4 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus Nectin-4, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
Background :
Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.
Synonyms :
EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4
References & Citations :
(1)Challita-Eid P M , Satpayev D , Yang P , et al. Enfortumab Vedotin Antibody-Drug Conjugate Targeting Nectin-4 is a Highly Potent Therapeutic Agent in Multiple Preclinical Cancer Models[J]. Cancer Research, 2016:0008-5472.CAN-15-1313.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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