Name : Human NGAL/Lipocalin-2 Protein
Product Source :
Recombinant Human NGAL/Lipocalin-2 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gln21-Gly198.[Accession | P80188-1]
Molecular Weight :
The protein has a predicted MW of 47.2 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in 50mM MES,150mM NaCl (pH 6.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM MES,150mM NaCl (pH 6.5).
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human NGAL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human NGAL is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human NGAL, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-NGAL Antibody, hFc Tag with the EC50 19.7ng/ml determined by ELISA.
Background :
Acute kidney injury (AKI) is one of the most common complications of various serious conditions, and early diagnosis is therefore critical for the treatment of AKI. Recent evidence demonstrates that neutrophil gelatinase- associated lipocalin (NGAL) is closely associated with AKI. Several experimental and clinical studies have shown that the expression of urine and serum NGAL increases significantly in AKI. NGAL shows potential to be a new effective early biochemical marker of AKI. Further studies are needed to confirm the significant advantages of NGAL in the diagnosis of early AKI and its value in clinical applications.
Synonyms :
NGAL; Lipocalin-2; Oncogene 24p3; p25; Siderocalin LCN2; MSFI
References & Citations :
(1) Shang W, Wang Z. The Update of NGAL in Acute Kidney Injury. Curr Protein Pept Sci. 2017;18(12):1211-1217. doi: 10.2174/1389203717666160909125004. PMID: 27634444.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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