Name : Biotinylated Human ACE2/ACEH Protein
Product Source :
Recombinant Biotinylated Human ACE2/ACEH Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Gln18-Ser740.[Accession | Q9BYF1-1]
Molecular Weight :
The protein has a predicted MW of 111.2 kDa. Due to glycosylation, the protein migrates to 115-125 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human ACE2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized SARS-COV-2 Spike S, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ACE2, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA.
Background :
ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension.The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.
Synonyms :
ACE-2; ACEH; ACE2
References & Citations :
(1)Kuba K , Imai Y , Rao S , et al. A crucial role of angiotensin converting enzyme 2 (ACE2) in SARS coronavirus–induced lung injury[J]. Nature Medicine, 2005, 11(8):875-879.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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