Name : Mouse Noggin Protein
Product Source :
Recombinant Mouse Noggin Protein is expressed from HEK293 without tag. It contains Gln28-Cys232.[Accession | P97466]
Molecular Weight :
The protein has a predicted MW of 23.07 kDa. Due to glycosylation, the protein migrates to 28-33 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse Noggin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse Noggin is greater than 95% as determined by SEC-HPLC. Cell Based Assay Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 4-80 ng/mL in the presence of 50 ng/mL of recombinant Human BMP‑4.
Background :
Noggin is an antagonist of bone morphogenetic proteins (BMP), being indispensable for certain developmental events.Noggin expression positively correlated with EGFR expression in both GC cell line models and The Cancer Genome Atlas human GC cohort. Targeting EGFR and its downstream pathways diminished cell proliferation which was promoted by Noggin. Noggin promotes the proliferation of GC cells by upregulating EGFR and enhancing a vicious circle formed by β‑catenin, EGFR, ERK and Akt.
Synonyms :
Noggin; NOG; SYM1; SYNS1
References & Citations :
(1) Sun Z, Gao X, Zabkiewicz C, Ruge F, Xie M, Cai S, Sun PH, Griffiths P, Pugh MR, Ji J, Jiang WG, Ye L. Noggin is associated with a poor prognosis of gastric cancer by promoting the proliferation of gastric cancer cells via the upregulation of EGFR. Int J Oncol. 2020 Sep;57(3):813-824. doi: 10.3892/ijo.2020.5081. Epub 2020 Jun 16. PMID: 32705152.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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