Name : Human Periostin/OSF-2 Protein
Product Source :
Recombinant Human Periostin/OSF-2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asn22-Gln836.[Accession | Q15063]
Molecular Weight :
The protein has a predicted MW of 92.1 kDa. Due to glycosylation, the protein migrates to 93-100 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Periostin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Periostin is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human Periostin, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.
Background :
Periostin is a matricellular protein that is expressed in several tissues during embryonic development; however, its expression in adults is mostly restricted to collagen-rich connective tissues. Periostin is expressed only briefly during kidney development, but it is not normally detected in the adult kidney. Recent evidence has revealed that periostin is aberrantly expressed in several forms of chronic kidney disease (CKD), and that its expression correlates with the degree of interstitial fibrosis and the decline in renal function.
Synonyms :
Periostin; PN; OSF-2; POSTN; OSF2; Fasciclin I-like; PDLPOSTN; PNRP11-412K4.1; TRIF52
References & Citations :
(1) Wallace DP. Periostin in the Kidney. Adv Exp Med Biol. 2019;1132:99-112. doi: 10.1007/978-981-13-6657-4_11. PMID: 31037629.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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