Name : Human OSMR Protein
Product Source :
Recombinant Human OSMR Protein is expressed from HEK293 with His tag at the C-terminus. It contains Glu28-Val236.[Accession | Q99650-1]
Molecular Weight :
The protein has a predicted MW of 25.09 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human OSMR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human OSMR is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human OSMR, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 15.1ng/ml determined by ELISA.
Background :
OSMR is targeted to the mitochondrial matrix via the presequence translocase-associated motor complex components, mtHSP70 and TIM44. OSMR interacts with NADH ubiquinone oxidoreductase 1/2 (NDUFS1/2) of complex I and promotes mitochondrial respiration. Deletion of OSMR impairs spare respiratory capacity, increases reactive oxygen species, and sensitizes BTSCs to IR-induced cell death.
Synonyms :
IL-31R subunit beta; IL-31R-beta; IL-31RB; OSMR beta; OSMR; OSMRB; OSMRBMGC150626; PLCA1 ;IL-31R subunit β; IL-31R-β; OSMR β
References & Citations :
(1) Sharanek A, Burban A, Laaper M, Heckel E, Joyal JS, Soleimani VD, Jahani-Asl A. OSMR controls glioma stem cell respiration and confers resistance of glioblastoma to ionizing radiation. Nat Commun. 2020 Aug 17;11(1):4116. doi: 10.1038/s41467-020-17885-z. PMID: 32807793; PMCID: PMC7431428.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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