Name : Human OX40 Ligand/TNFSF4 Protein
Product Source :
Recombinant Human OX40 Ligand/TNFSF4 Protein is expressed from HEK293 with hFc tag at the N-terminus. It contains Gln51-Leu183.[Accession | P23510-1]
Molecular Weight :
The protein has a predicted MW of 40.95 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human OX40 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human OX40 Ligand is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Biotinylated Human OX40, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA (QC Test). SPR Data Human OX40, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human OX40 Ligand, hFc Tag with an affinity constant of 4.17 nM as determined in SPR assay (Biacore T200).
Background :
Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation.
Synonyms :
OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L; TXGP1; Glycoprotein Gp34
References & Citations :
(1)Al-Shamkhani A , Mallett S , Brown M H , et al. Affinity and Kinetics of the Interaction between Soluble Trimeric OX40 Ligand, a Member of the Tumor Necrosis Factor Superfamily, and Its Receptor OX40 on Activated T Cells[J]. Journal of Biological Chemistry, 1997, 272(8):5275-5282.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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