Name : Human IL-12B/p40/NKSF2 Protein
Product Source :
Recombinant Human IL-12B/p40/NKSF2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ile23-Ser328.[Accession | P29460]
Molecular Weight :
The protein has a predicted MW of 34.7 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-12B on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human IL-12B, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA (QC Test). ELISA Data Immobilized Human IL-12B, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-12 R beta 1, hFc Tag with the EC50 of 0.26μg/ml determined by ELISA. SPR Data Human IL-12B, His Tag immobilized on CM5 Chip can bind Human IL-12 R beta 1, His Tag with an affinity constant of 98.1nM as determined in a SPR assay (Biacore T200).
Background :
Interleukin (IL)‑12B, which encodes the p40 subunit common to IL‑12 and IL‑23, as one of the genes for which expression in fibroblast‑like synoviocytes from patients with rheumatoid arthritis (RA‑FLS) is induced by DcR3.Decoy receptor 3 (DcR3) competitively binds to three ligands, Fas ligand, lymphotoxin‑related inducible ligand that competes for glycoprotein D binding to herpesvirus entry mediator on T cells and tumor necrosis factor‑like ligand 1A (TL1A), to prevent their effects. Recent studies have suggested that DcR3 directly affects cells as a ligand.
Synonyms :
IL-12B; CLMF p40; IL-12 subunit p40; NKSF2; CLMF; CLMF2; NKS
References & Citations :
(1) Fukuda K, Miura Y, Maeda T, Hayashi S, Kurosaka M. Interleukin‑12B is upregulated by decoy receptor 3 in rheumatoid synovial fibroblasts. Mol Med Rep. 2016 Apr;13(4):3647-52. doi: 10.3892/mmr.2016.4985. Epub 2016 Mar 7. PMID: 26956410.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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