Name : Cynomolgus uPAR/PLAUR Protein
Product Source :
Recombinant Cynomolgus uPAR/PLAUR Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu23-Gly305.[Accession | Q9GK78]
Molecular Weight :
The protein has a predicted MW of 32.69 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus uPAR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus uPAR is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus uPAR, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test). SPR Data Cynomolgus uPAR, His Tag immobilized on CM5 Chip can bind Cynomolgus PLAU, His Tag with an affinity constant of 0.033 nM as determined in SPR assay (Biacore T200).
Background :
The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR.
Synonyms :
U-PAR; uPAR; CD87; PLAUR; MO3; UPAR
References & Citations :
(1) Blasi F. The urokinase receptor and cell migration. Semin Thromb Hemost. 1996;22(6):513-6. doi: 10.1055/s-2007-999053. PMID: 9122717.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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