Name : Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein
Product Source :
Recombinant Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Phe19-Arg238.[Accession | G7PSE7]
Molecular Weight :
The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus/Rhesus macaque PD-L1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus/Rhesus macaque PD-L1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus/Rhesus macaque PD-L1, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test). SPR Data Cynomolgus PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus/Rhesus macaque PD-L1, His Tag with an affinity constant of 1.28 μM as determined in SPR assay (Biacore T200).
Background :
B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
Synonyms :
CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1
References & Citations :
(1)Blank C , Mackensen A. Contribution of the PD-L1/PD-1 pathway to T-cell exhaustion: an update on implications for chronic infections and tumor evasion[J]. Cancer Immunology Immunotherapy, 2007, 56(5):739-745.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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