Name : Rat PDGF-AA Protein
Product Source :
Recombinant Rat PDGF-AA Protein is expressed from Yeast with His tag at the N-terminus. It contains Arg86-Lys204.[Accession | P28576-1]
Molecular Weight :
The protein has a predicted MW of 14.75 kDa. Due to glycosylation, the protein migrates to 17-22 kDa and 25-45 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 90% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Rat PDGF-AA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. Cell Based Assay Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is 5 – 15 ng/mL.
Background :
PDGF (Platelet-derived growth factor) is one among numerous growth factors that regulate cell growth and division. The PDGF family forms five dimeric ligands, PDGF-AA, PDGF-BB, PDGF-CC, PDGF-DD, and PDGF-AB, which activate cognate receptor tyrosine kinases PDGFR-α and PDGFR-β. Upon binding to its tyrosine kinase receptor PDGF-αα, PDGF-AA stimulates the proliferation of osteoblastic cells and may exert autocrine and paracrine effects in regulating bone-forming processes. PDGF-AA is the most well characterized mitogen that is not restricted to any kind of tumor cell type but was seen in osteoblastic cells as well as in pleomorphic tumor elements.
Synonyms :
PDGF-AA; PDGF-1; Pdgfa; Rpa1;
References & Citations :
[1] Sulzbacher I, Träxler M, Mosberger I, Lang S, Chott A. Platelet-derived growth factor-AA and -alpha receptor expression suggests an autocrine and/or paracrine loop in osteosarcoma. Mod Pathol. 2000 Jun;13(6):632-7.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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