Name : Human PGLYRP1 Protein
Product Source :
Recombinant Human PGLYRP1 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gln22-Pro196.[Accession | O75594]
Molecular Weight :
The protein has a predicted MW of 46.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human PGLYRP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human PGLYRP1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human TREM1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human PGLYRP1, hFc Tag with the EC50 of 0.32μg/ml determined by ELISA.
Background :
Innate immunity protein Tag7 (PGRP-S, PGLYRP1) can interact with the TNFα receptor, TNFR1, and block the transduction of apoptotic signals through this receptor. A complex formed between the Tag7 protein and the major heat shock protein Hsp70 can activate TNFR1 receptor and induce tumor cell death via either apoptotic or necroptotic pathway.
Synonyms :
PGRP-S; PGLYRP1; PGLYRP; PGRP; TNFSF3L; PGRP-SMGC126894; PGRPSMGC126896; TAG7; Tasg7
References & Citations :
(1) Romanova EA, Sharapova TN, Telegin GB, Minakov AN, Chernov AS, Ivanova OK, Bychkov ML, Sashchenko LP, Yashin DV. A 12-mer Peptide of Tag7 (PGLYRP1) Forms a Cytotoxic Complex with Hsp70 and Inhibits TNF-Alpha Induced Cell Death. Cells. 2020 Feb 20;9(2):488. doi: 10.3390/cells9020488. PMID: 32093269; PMCID: PMC7072780.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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