Name : Human PLXNA1 Protein

Product Source :
Recombinant Human PLXNA1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu27-Pro1244.[Accession | Q9UIW2]

Molecular Weight :
The protein has a predicted MW of 135 kDa. Due to glycosylation, the protein migrates to 140-155 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human PLXNA1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human PLXNA1 is greater than 95% as determined by SEC-HPLC.

Background :
PLXNA1 (Plexin A1) is a member of plexin family that are the receptors of semaphorins. It is involved in cell migration and neuronal repulsion by affecting cytoskeletal remodeling. PLXNA1 can bind class 6 semaphorins and bind the class 3 semaphorins indirectly via Neuropilin-1 and -2.

Synonyms :
Plexin A1, Semaphorin receptor NOV, NOV, PLXN1

References & Citations :
(1) Kumanogoh A, Kikutani H. Immunological functions of the neuropilins and plexins as receptors for semaphorins. Nat Rev Immunol. 2013 Nov;13(11):802-14. doi: 10.1038/nri3545. PMID: 24319778.

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