Name : Biotinylated Mouse BCMA/TNFRSF17 Protein
Product Source :
Recombinant Biotinylated Mouse BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Met1-Thr49.[Accession | O88472-1]
Molecular Weight :
The protein has a predicted MW of 8.5 kDa. Due to glycosylation, the protein migrates to 12-16 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Mouse BCMA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Mouse BCMA is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Mouse APRIL, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA (QC Test). SPR Data Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Mouse BCMA, His-Avi Tag with an affinity constant of 5.64 μM as determined in SPR assay (Biacore T200).
Background :
B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF).
Synonyms :
CD269; TNFRSF17; BCMA; BCM; TNFRSF13A
References & Citations :
(1)Bossen C , Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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