Name : Human/Murine/Rat BDNF Protein
Product Source :
Recombinant Human/Murine/Rat BDNF Protein is expressed from E.coli without tag It contains His129-Arg247.[Accession | P23560-1]
Molecular Weight :
The protein has a predicted MW of 13.51 kDa. The protein migrates to 15-19 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human/Murine/Rat BDNF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human/Murine/Rat BDNF, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.17μg/ml determined by ELISA.
Background :
The brain-derived neurotrophic factor (BDNF) is a secretory growth factor that promotes neuronal proliferation and survival, synaptic plasticity and long-term potentiation in the central nervous system. Brain-derived neurotrophic factor biosynthesis and secretion are chrono-topically regulated processes at the cellular level, accounting for specific localizations and functions.
Synonyms :
Abrineurin; BDNF; ANON2; BULN2
References & Citations :
Di Carlo P, Punzi G, Ursini G. Brain-derived neurotrophic factor and schizophrenia. Psychiatr Genet. 2019 Oct;29(5):200-210. doi: 10.1097/YPG.0000000000000237. PMID: 31465000; PMCID: PMC7386257.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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