Name : Human PRNP Protein
Product Source :
Recombinant Human PRNP Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Lys23-Ser230.[Accession | P04156]
Molecular Weight :
The protein has a predicted MW of 49.4 kDa. Due to glycosylation, the protein migrates to 60-67 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human PRNP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human PRNP, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-PRNP Antibody, hFc Tag with the EC50 of 33.2ng/ml determined by ELISA.
Background :
Prion protein gene (PRNP) variants determine the susceptibility of humans, sheep and mice to prion diseases, whereas polymorphisms in the open reading frame (ORF) of bovine PRNP seem to be unrelated to the incidence of bovine spongiform encephalopathy (BSE). According to the latest reports, the genetic susceptibility of cattle to BSE is associated with polymorphisms ofthe regulatory region of the PRNP gene and the level ofits expression.
Synonyms :
Major prion protein;PrP;ALTPRP;PRIP;PRP;PRNP
References & Citations :
(1) Gurgul A, Słota E. Effect of bovine PRNP gene polymorphisms on BSE susceptibility in cattle. Folia Biol (Krakow). 2007;55(3-4):81-6. doi: 10.3409/173491607781492533. PMID: 18274249.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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