Name : Mouse PSGL-1 Protein

Product Source :
Recombinant Mouse PSGL-1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln42-Cys307.[Accession | Q62170]

Molecular Weight :
The protein has a predicted MW of 28.74 kDa. Due to glycosylation, the protein migrates to 68-108 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Mouse PSGL-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse PSGL-1 is greater than 95% as determined by SEC-HPLC.

Background :
P-Selectin glycoprotein ligand-1 (PSGL-1) has long been studied as an adhesion molecule involved in immune cell trafficking and is recognized as a regulator of many facets of immune responses by myeloid cells. PSGL-1 also regulates T cell migration during homeostasis and inflammatory settings.

Synonyms :
CLA; PSGL-1; PSGL1; CD162; SELPLG; Selectin P ligand; Selp1; Selpl

References & Citations :
(1) Tinoco R, Otero DC, Takahashi AA, Bradley LM. PSGL-1: A New Player in the Immune Checkpoint Landscape. Trends Immunol. 2017 May;38(5):323-335. doi: 10.1016/j.it.2017.02.002. Epub 2017 Mar 2. PMID: 28262471; PMCID: PMC5411281.

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