Name : Mouse PSAP/Prosaposin Protein
Product Source :
Recombinant Mouse PSAP/Prosaposin Protein is expressed from HEK293 with His tag at the C-terminus. It contains Ser17-Asn557.[Accession | Q61207]
Molecular Weight :
The protein has a predicted MW of 60.86 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse PSAP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
Prosaposin (also known as SGP-1) is an intriguing multifunctional protein that plays roles both intracellularly, as a regulator of lysosomal enzyme function, and extracellularly, as a secreted factor with neuroprotective and glioprotective effects.
Synonyms :
SGP-1; SGP1; Sgp1
References & Citations :
Meyer RC, Giddens MM, Coleman BM, Hall RA. The protective role of prosaposin and its receptors in the nervous system. Brain Res. 2014 Oct 17;1585:1-12. doi: 10.1016/j.brainres.2014.08.022. Epub 2014 Aug 15. PMID: 25130661; PMCID: PMC4529117.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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