Name : Human Activin A Protein
Product Source :
Recombinant Human Activin A Protein is expressed from HEK293 without tag. It contains Gly311-Ser426.[Accession | P08476]
Molecular Weight :
The protein has a predicted MW of 12.97 kDa. Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 23-25 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 0.1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in 4mM HCI. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCI.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Activin A on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%. ELISA Data Immobilized Human Activin A, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human Activin RIIB, His Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test). ELISA Data Immobilized Human Activin A, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human Activin RIIA, His Tag with the EC50 of 0.23μg/ml determined by ELISA. Cell Based Assay Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is
Background :
Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression.
Synonyms :
INHBA; Activin A;
References & Citations :
Morianos I, Papadopoulou G, Semitekolou M, Xanthou G. Activin-A in the regulation of immunity in health and disease. J Autoimmun. 2019 Nov;104:102314. doi: 10.1016/j.jaut.2019.102314. Epub 2019 Aug 13. PMID: 31416681.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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